Ion AmpliSeq™ On‑Demand Panels

Ion AmpliSeq™ On-Demand designs are appropriate for common disease research areas and are customizable at the gene level. The designs include optimized amplicons with verified performance for germline analysis. You can create designs by selecting from a catalog of > 5,000 pre‑tested genes or browsing the Ion AmpliSeq™ Designer gene repository by research area of interest.

Each Ion AmpliSeq™ On‑Demand Panel consists of 2 DNA primer pools, where the number of primer pairs per pool depends on the genes that are selected. The maximum number of genes that can be selected for design creation is 800. Each Ion AmpliSeq™ On‑Demand panel order is limited to 500 genes or 15,000 amplicons. The minimum number of amplicons for Ion AmpliSeq™ On‑Demand designs is 12 amplicons per pool (or 24 amplicons per design).

You can add genes that are not in the On-Demand catalog to a panel as a Spike‑in Panel. Spike-in Panels are high concentration Made-To-Order panels that are used to supplement On‑Demand designs with genes that are not available On-Demand. Spike‑in Panels are created as 2‑pool designs and are limited to 123 amplicons per pool.

Ion AmpliSeq™ On-Demand panels are available in 8 or 32 reaction packs for library preparations on the Ion Chef™ System and 24 or 96 reaction packs for manual library preparation. The panels support sequencing genomic targets from as little as 10 ng of DNA input using the Ion PGM™ System.

To create a new Ion AmpliSeq™ On‑Demand Panel, start a new design using one of the following methods, then proceed to Order an Ion AmpliSeq On‑Demand Panel.

• To start a new design by manually entering a custom gene list or uploading a list of genes using a CSV file, see Start a new Ion AmpliSeq On-Demand design by manually entering a list of genes or uploading a CSV file.

• To start a new design by selecting genes using a content selection engine, see Start a new Ion AmpliSeq On-Demand design by browsing disease research areas.