Ion AmpliSeq™ Custom panels

Custom panels are customizable for any genome at the amplicon level. Panels can be designed against many pre-loaded reference genomes, or for any other organism by uploading a FASTA file reference sequence.

Custom panels are delivered within a few weeks in tubes of pre-pooled, multiplexed primers in ready-to-use concentrations. You can add 384‑well plates to each DNA custom panel order, where each well contains individual primer pairs in concentrated format.

Table 1. Number of reactions for Custom panels
Nucleic acid type	Format	Number of amplicons	Reactions^[1]
DNA	Tubes Only	≤96	750
	Tubes Only	>96	3,000
	Tubes Plus 384-Well Plate	≤96	2,250 total 750 in pools 1,500 in plate format
	Tubes Plus 384-Well Plate	>96	9,000 total 3,000 in pools 6,000 in plate format
RNA	Tubes Only	any	1,125

¹ The number of reactions listed here are for manual library preparation in a 20 μL target amplification volume. For more information about the number of reactions for each library preparation method, reaction volume, and panel-specific recommendations, see the Ion AmpliSeq™ User Guides. For a list of User Guides, see Related documentation.

To create a new Ion AmpliSeq™ Custom panel, start a new design using one of the following methods, then proceed to Order an Ion AmpliSeq custom panel.

To start a new DNA Gene design, see Start a new Ion AmpliSeq DNA Gene design.
To start a new DNA Hotspot design, see Start a new Ion AmpliSeq DNA Hotspot design.
To start a new RNA Gene Expression design, see Start a new Ion AmpliSeq RNA Gene Expression design.
To start a new RNA Gene Fusion design, see Start a new Ion AmpliSeq RNA Gene Fusion design.

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Ion AmpliSeq™ Custom panels