Ion AmpliSeq™ Custom panels: RNA Gene Expression design input specifications

For Ion AmpliSeq™ Custom RNA gene expression designs, the following input is case-sensitive.

• The names of the genes and accession numbers are required to use capital letters.

• The RNA_REFSEQ and RNA_GENE labels require to use capital letters.

• Gene symbols must follow the HUGO nomenclature. The HGNC standard for human gene symbols requires the use of all capital letters.

• The header Type,Name in the CSV file is optional and case insensitive.

To add multiple targets at one time, you can use a CSV or a Gene List file. The following input files are accepted for Ion AmpliSeq™ RNA gene expression designs. To view example files, in the target addition pane, click Input Specifications, then click Download this example for each file type of interest. You can copy/edit the example files to create panel‑specific input files.