Ion Chip capacities for Ion AmpliSeq™ DNA libraries sequenced at equal depths
The number of combined libraries that can be accommodated in a single sequencing run depends on the chip, the balance of barcoded library concentration, and the coverage required.
For a given chip, as the number of amplicons increases, the number of libraries that can be accommodated per sequencing run decreases. This relationship is shown in the following table. The numbers in the following table serve as a guide for approximate capacities. As the number of libraries per chip increases, it becomes more difficult to balance the reads between libraries. In addition, libraries from FFPE tissue tend to produce more variable results. We suggest combining fewer libraries initially and determining real limits empirically.
|
Chip |
Ion 314™[1] |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Average depth |
150X |
2500X |
150X |
2500X |
150X |
2500X |
150X |
2500X |
150X |
2500X |
150X |
2500X |
|
Expected coverage |
95% |
95% |
95% |
95% |
95% |
95% |
95% |
95% |
95% |
95% |
95% |
95% |
|
Number of amplicons per library |
Approximate number of libraries per chip |
|||||||||||
|
12–24 |
84 |
8 |
272 |
26 |
>384 |
48 |
>384 |
152 |
>384 |
>384 |
>384 |
>384 |
|
25–48 |
48 |
4 |
152 |
14 |
272 |
26 |
>384 |
84 |
>384 |
272 |
>384 |
>384 |
|
49–96 |
26 |
2 |
84 |
8 |
152 |
14 |
>384 |
48 |
>384 |
152 |
>384 |
243 |
|
97–192 |
14 |
1 |
48 |
4 |
84 |
8 |
272 |
26 |
>384 |
84 |
>384 |
134 |
|
193–384 |
8 |
— |
26 |
2 |
48 |
4 |
152 |
14 |
>384 |
48 |
>384 |
77 |
|
385–768 |
4 |
— |
14 |
1 |
26 |
2 |
84 |
8 |
272 |
26 |
>384 |
41 |
|
769–1,536 |
2 |
— |
8 |
— |
14 |
1 |
48 |
4 |
152 |
14 |
243 |
22 |
|
1,537–3,072 |
1 |
— |
4 |
— |
8 |
— |
26 |
2 |
84 |
8 |
134 |
13 |
|
3,073–6,144 |
— |
— |
2 |
— |
4 |
— |
14 |
1 |
48 |
4 |
77 |
7 |
|
6,145–12,288 |
— |
— |
1 |
— |
2 |
— |
8 |
— |
26 |
2 |
41 |
3 |
|
12,289–24,576 |
— |
— |
— |
— |
1 |
— |
4 |
— |
14 |
1 |
22 |
2 |
|
24,577–49,152 |
— |
— |
— |
— |
— |
— |
2 |
— |
8 |
— |
13 |
1 |