Ion AmpliSeq™ Designer filters regions that have either very high or very low GC content.
Using the UCSC Table Browser, select the Mapping and Sequencing Tracks group option and the GC Percent track option.
In the Enter region definition dialog box, you can enter or paste a region definition to search for a specific region, or upload your designed BED file to the UCSC Table Browser.
The output appears in a 5-basepair sliding window where you can determine regions that are below 20% or above 80% in GC content.
