Create a custom BED file for input into Ion AmpliSeq™ Designer

You can generate a custom BED file from the list of variants with your target regions of interest using the UCSC Genome Browser, then import the BED file into Ion AmpliSeq™ Designer.

• To create a BED-formatted file starting from a list of dbSNP identifiers, use the UCSC Table Browser.

• To generate a custom BED file from a list of COSMIC mutations, use the COSMIC database at https://cancer.sanger.ac.uk/cosmic.